Program Streams and Symposia Topics
IMC19 represents a forum for sharing and contesting the latest ideas and technologies in the world of microscopy. The 2018 program will be truly transformational, featuring the world’s thought leaders and rising stars.
The scientific program will include world-renowned plenary and invited speakers, young scientists showcasing their research, digital posters and Pre-Congress workshops. The program will encompass four main streams – Life Sciences, Physical Sciences, Instrumentation and Techniques and Frontier Issues.
Chair: Prof Julie Cairney – The University of Sydney, Australia
Committee: A/Prof Jodie Bradby, Prof Marc de Graef, Dr Alex la Fontaine, Prof Dr Joachim Mayer, A/Prof Matt Weyland, Prof Jin Zou
PS1 - Nanoscale, nanostructured and porous materials
Chairs: Ben Britton and Frances M. Ross
Electron microscopy is an indispensable tool for structural characterization and local property measurements of both nanostructured materials and nanoscale objects. This symposium aims to highlight the applications of electron and other microscopies to analyse these materials, and to demonstrate how information obtained facilitates the development of new types of materials that are nanostructured, nanoporous or have hierarchical architectures. We will discuss microscopy of nanostructured metallic, ceramic, and organic materials, nanoparticles such as nanowires, and materials with nanoscale porosity. We welcome contributions that make use of all types of microscopy to measure such materials. Understanding local chemical and crystallographic structure and porosity in two or three dimensions may require advanced S/TEM or SEM. Structure-property correlations may be measured by in situ techniques such as mechanical testing or electrical probing. The use of correlative methods that combine information from electron microscopy with atom probe or SIMS is particularly relevant for nanostructured and nanoscale materials. We anticipate an exciting forum to discuss the current state and future opportunities for microscopy to understand the properties and improve the performance of nanoscale materials.
PS2 - Carbon-based materials and 2D structures
Chairs: Ute Kaiser & Dougal McCulloch
Carbon-based nanomaterials, such as carbon nanoparticles, nanotubes, fullerenes and graphenes are well established in the nanosciences. Engineering applications are growing extremely fast, with microscopy playing a pivotal role. Conversely, rapid progress in the nanosciences has been driving the latest developments in microscopic instrumentation and techniques. This symposium focuses on microscopic investigation of carbon-based nanomaterials and nanostructured diamond thin films, including the determination of their atomic and chemical structure, characterization of their electronic, magnetic and optical properties, and observation and characterization of their dynamic behaviour under various external interventions.
PS3 - Thin films, coatings and surfaces
Chairs: Xiuliang Ma & Zonghan Xie
Special microstructures found at material surfaces, coatings and thin films play an essential role in a number of research domains, and microscopic imaging and analytical techniques have become indispensable for their investigation. This symposium highlights the use of electron microscopy techniques in surface and thin film research. The issues to be covered include characterization of defects at interfaces, assessment of composition and electronic bonding at interfaces using HAADF-STEM and EELS, correlation of theory/simulation with experimental measurements, investigation of special interfaces between dissimilar materials essential in electronics, medical and smart materials applications.
PS4 - Metals and alloys
Chairs: Xiaodong Han & Jianfeng Nie
Novel advanced engineering metallic materials and metal-matrix composites are being developed in response to ongoing demand from industry for higher strength, lower weight and improved fatigue and corrosion resistance. Significant breakthroughs in engineering material research and development require detailed knowledge of composition-processing-structure-property relationships. Recent advances in spatial and chemical resolution of electron microscopy techniques, along with high-resolution 3D information from atom probe techniques, allows for a more detailed investigation into the composition-processing-structure-property relationships in metals and alloys, including compositionally highly complex advanced engineering materials, multiphase nanostructured steels, materials having undergone severe plastic deformation, materials hardened by precipitates etc. This symposium encompasses applications of modern microscopic imaging and spectroscopy methods and the multi-scale (from atomic to micron) investigation of microstructures in metallic alloys and metal-matrix composites.
PS5 - Ceramics and inorganic composites
Chairs: Ikuhara Yuichi & Peter Crozier
– Stephen Pennycook, Singapore
– Gianluigi Botton, Canada
– Susanne Stemmer, U.S.
– Naoya Shibata, Japan
Lattice defects such as point defects and grain boundaries in advanced ceramics and inorganic composites have peculiar atomic and electronic structures, which are caused by the disorder in periodicity, providing the mechanical and functional properties. It is very important to determine the structures, chemical composition and electronic state to understand the mechanism to provide the properties. For this purpose, TEM, STEM, diffraction contrast, EDS, EELS, electron holography, DPC are useful methods to characterize the lattice defects in ceramics. For example, in the vicinity of grain boundaries, dopants or impurities are often segregated, and they play a crucial role in deciding the properties of ceramics. In this case, Cs corrected STEM, allowing the formation of sub-angstrom-sized electron probes, can directly observe grain boundary-segregated dopants. In this session, the followings topics will be discussed for advanced ceramics and inorganic composites.
(1) Grain boundary and interface characterization
(2) Lattice defects, dopant, point defects
(3) Diffraction contrast to characterize lattice defects
(4) Cs corrected STEM and TEM characterization
(5) Advanced imaging techniques for advanced ceramics
(6) Diffusion, Phase transformation, Dynamic behavior
(7) Energy materials, Oxides, Carbides, Nitrides and so on
PS6 - Biomaterials, polymers and polymer-based composites
Chairs: Hala Zreiqat & Cheng Yan
Synthetic polymers, biopolymers, and their composites with inorganic materials represent a unique kind of material for microscopic studies. They are structured over a wide range of length scales from the macroscale to nanoscale. A variety of imaging methods such as optical microscopy, SEM, TEM, SPM, X-ray and neutron imaging is thus required. In addition, the development of materials for biomedical applications, such as implants or external biomedical devices, is an area of growth in materials science that frequently involves advanced microscopy.
PS7 - Semiconductors and materials for communication
Chairs: Dave Muller & Jenny Wong-Leung
Microscopy techniques are currently heavily used in semiconductor research for applications in microelectronics, optoelectronics, memristors, photovoltaics, bioelectronics, quantum computing and others. In particular electron microscopy is an indispensable technique for nanotechnology with major advances in aberration corrected electron microscopy becoming indispensable in understanding the properties of semiconductor nanostructures and their potential applications. This symposium invites contributions about microscopy methods as applied to the investigation of conventional and new semiconductor materials.
PS8 - Phase transformations and corrosion
Chairs: David Young & Jim Howe
The phases that make up materials govern the properties of materials. Advanced microscopy has historically played a pivotal role in expanding our knowledge about structure, chemical composition and physical properties of phases and how they form and transform. In-situ microscopy is leading to advancements in knowledge in this area. Corrosion processes are also governed by these transformations. This symposium will concentrate on research around understanding phase transformations and corrosion via microscopy techniques.
PS9 - Amorphous and disordered materials, liquid crystals
Chairs: Amelia Liu & Paul Voyles
Microscopy is a powerful tool for the investigation of the structure of amorphous and disordered materials (semiconductors, ceramics, metallic glasses, network glasses, colloidal dispersions, liquid crystals and polymers). These complex materials are extremely challenging to characterise, and require novel methods and analysis techniques. The symposium invites contributions about microscopic characterization of structurally disordered materials by imaging, diffraction and spectroscopy methods, as well as reports of advances in determining structure-properties relationships. Materials featuring disorder that is not purely structural, such as polarization, magnetization, or spin domains are not within the scope of this symposium.
PS10 - Magnetic, ferroelectric and multiferroic materials
Chairs: Shujun Zhang & Laura Bocher
– Christophe Gatel, France
Magnetic, ferroelectric and multiferroic functional materials for information technologies and smart structure applications are set to remain in the limelight to design and engineer the new types of electronic devices. The “non-structural disorder” in these materials manifests itself as highly mobile domains and domain walls. These are due to a local arrangement of magnetization and polarization. In many of these materials, the peculiar functionality issues from their unique interplay between atomic and electronic structures both at the scale of an atomic column and at correlation lengths of tens to hundreds of nanometers. This symposium focuses on the latest applications of electron microscopy (EM) techniques to characterize local structure and magnetic, electric and/or electronic properties of these materials. We welcome contributions reporting either new technique advances relying on image-based tools, spectroscopic imaging, holography techniques, in-situ modes e.g. temperature- and field-induced phase transitions and/or novel applications exploiting domain walls, 2D lattices of skyrmions and polar structures among others. Contributions highlighting the combination between EM-based and synchrotron-based techniques (e.g. XMCD, PEEM) are encouraged further as well as contributions where theoretical or modeling work is bringing an original asset.
PS11 - Materials in geology, mineralogy and archeology
Chairs: David Saxey
Microscopy has traditionally been an indispensable tool in investigating geological materials. Recent improvements in resolution of electron microscopes have opened up new possibilities for the characterization of geological materials at the nanoscale level (HRTEM/HRSTEM), including crystallographic (ED, NBD, CBED) and microchemical (EDS, EELS, EFTEM) characterization. This symposium focuses on a microscopic characterization of geomaterials from a wide range of research areas, including traditional earth sciences.
PS12 - Materials for energy production, storage and catalysis
Chairs: Christina (Tina) Scheu
Due to the accelerated diminishing of natural resources, materials required for the generation, transmission and storage of energy, such as those employed in nuclear power plants, fuel and solar cells, and hydrogen storage or thermoelectric devices are of increased importance. Research into and development of new catalytic materials required in novel clean technologies for environmentally friendly energy generation, nanoelectronics and healthcare applications has already undergone a significant shift towards nanomaterials, thus heavily relying on modern microscopic methods. This symposium focuses on the applications of advanced microscopy and spectroscopy methods to study materials for energy conversion and storage or catalysis.
PS13 - Physical science applications of in-situ microscopy
Chairs: Masaki Takeguchi & Xiaozhou Liao
– Dmitri Golberg, Australia
– Nigel Browning, UK
– Zibin Chen, Australia
– Julia R. Greer, U.S.
In-situ transmission electron microscopy techniques are moving beyond first-of-their-kind demonstrations in the lab to becoming mainstream microscopy techniques being used to solve real-world materials problems. This symposium will focus on physical science applications of in-situ microscopy, rather than focusing on developments in in-situ instrumentation.
Instrumentation and Techniques
Chair: Prof Joanne Etheridge – Monash University, Australia
Committee: Prof Michael Bosman, Prof Wah Chiu, Prof Jacob Hoogenboom, Prof Sandra Van Aert, Prof Roger Wepf
IT1 – Instrumentation
Chairs: Greg McMullan & Nestor Zaluzec
Technological breakthroughs for both imaging and spectroscopy using electron, ion, photon and x-rays are dramatically advancing our ability to characterize materials in both the physical and life sciences. The session welcomes both experimental as well as theoretical contributions in the area of instrumentation, methodology and software which advances our ability to characterize both hard and soft matter in all regimes. Topical areas include: sources, structured illumination, adaptive optics, aberration correctors, spectrometers, detectors, as well as ancillary technology particularly related to cryo and in-situ experiments.
IT2 - Computational methods for data acquisition, analysis and visualization
Chairs: Steven Ludtke & Nigel Browning
Recent developments within each microscopy modality have produced significant advances in spatial and temporal resolution, precision, contrast and throughput. Improvements in detectors and associated computer hardware now permit massive quantities of data to be produced, and it is becoming increasingly clear that acquiring an image, spectrum, or tomogram is only the first step in knowledge generation. The statistical, mathematical and computational methods to analyze large numbers of noisy images and manipulate large complex datasets are also being developed at an unprecedented pace. The goal of this symposium is to provide a forum to discuss how novel mathematical and computational concepts can be adapted to microscopy workflows to yield new information from the vast quantities of images now produced in the various microscopy disciplines. A few of the novel topics to be discussed include: compressive sensing, real-time/high-throughput image analysis, deep learning and related neural network techniques, bispectral analysis, and the use of emerging computational hardware including GPUs, FPGAs and dedicated neural processing chips. Contributions are welcome in any area of emerging computational methods for processing microscopy data.
IT3 – Methods and workflows for correlative microscopy
Chairs: Fei Sun & Saskia Lippens
This session will focus on developments in the correlative microscopy field. Correlative microscopy is a methodology that allows to image the same specimen with multiple microscopic approaches, generate different sources of image data from the same specimen and correlate them together into the same coordinate. The result is a multi-dimensional (multi-mode) image that provides both specific and comprehensive information of the specimen. It includes correlative fluorescence and electron microscopy (CLEM), cryo-CLEM, correlation of fluorescence and atomic force microscopy, correlation of mass spectrum and scanning electron microscopy, correlation of Raman and atomic force microscopy, correlation of florescence and X-ray microscopy, and many other techniques. In recent years, the field of correlative microscopy has made essential progresses and the new emerging techniques will have a big impact for the future of research life, medical and materials sciences. This session will discuss the recent developments of correlative microscopy, from specimen preparation and probe development, to experimental workflow, new hardware and software, to the final image analysis and data management.
IT4 - Cryo-TEM techniques for biological material
Chairs: Juergen Plitzko & Christopher Russo
Please note the chairs are firming the session description below:
Developments in techniques, including cryo-specimen preparation (including cryo-FIB), data collection, image processing, modeling and structure validation. This session covers advances in single particle cryo-electron microscopy at atomic resolution and cryo-electron tomography at subnanometer resolution.
IT5 - In-situ, environmental and time-resolved microscopies
Chairs: Patricia Kooyman & Pratihba Gai
The symposium will cover developments and applications of in-situ, environmental, scanning probe and fast time resolved studies of increasing interest. It will focus on the pivotal role of imaging and spectroscopy of dynamic processes across the sciences to access previously invisible aspects of real world processes and complementing and extending examinations of static structures. Advances in high speed recording, novel stage holder designs and sample preparation methods are advancing nanostructure analyses in gases and liquids; often combined with other controlled conditions of temperature, electrical and beam parameters. The real time studies of materials in change-inducing environments lead to a new understanding as the basis for development of properties on the nanometre and atomic scale. They contribute to the full range of materials sciences and technologies (including in catalysis, semiconductors, carbons, nanoelectronics, biomaterials and energy) in diverse applications of vital importance to industry, society and the environment, but often inaccessible with conventional microscopies. Both platform and poster contributions are welcome to supplement invited talks.
IT6 -Diffraction techniques
Chairs: Randi Holmestad & Kenji Tsuda
Developments in diffraction techniques, including experimental methods, theory and simulations. The session will cover all radiation types, including electrons, light, and x-rays, including XFELS. Topics include crystallography, nanodiffraction, diffraction with cold-atom sources, CBED, LACBED, EBSD, scanning diffraction, precession diffraction, time resolved diffraction, coherent diffraction and powder diffraction.
IT7 - Multi-scale 3D imaging
Chairs: Paul Matsudaira & Sara Bals
Developments in techniques, including experimental methods, reconstruction algorithms (scalar and vector) and specimen preparation. Session covers light, electron and x-ray techniques. Topics include electron tomography, x-ray tomography and nano-CT, micro-CT, confocal microscopies (light and electron), serial sectioning (light, electron and ion beam), multiphoton microscopy, adaptive optics, light sheet, clearing, optical projection tomography and low dose techniques. Excludes cryo-EM tomography of biological materials which should go IT4 – Cryo-TEM techniques for biological material.)
IT8 - Phase-related techniques
Chairs: Etienne Snoeck & Nobuo Tanaka
The session covers various kinds of analysis methods of structures and properties of matters by electron, X-ray and other beams in viewpoint of use of phase rather than amplitude of waves. Those include TEM and STEM holography, diffraction imaging, ptychography, lens-less imaging, structured illumination, differential phase contrast method, transmission intensity equation, and exit-wave reconstruction for biological and non-biological samples. The chairs encourage young scientists for innovative researches from all of the contribution to the present session of IMC19. Joint sessions will be considered with IT9: STEM and TEM imaging
IT9 - STEM and TEM imaging
Chairs: Richard Leapman & Peter Nellist
Imaging is at the heart of electron microscopy. In STEM and TEM imaging, recent developments have included methods for precise quantification of images, data processing approaches that improve precision and reduce the electron dose required, new detector technologies for higher speed and improved detection efficiency, applications of aberration correction and novel imaging configurations. In this session, we encourage submissions that demonstrate developments and applications in TEM and STEM imaging, in particular those that are at the frontiers of what is currently possible.
IT10 - SEM, FIB, scanning probe and surface microscopy
Chairs: Tomonobu Nakayama & Raynald Gauvin & Alex de Marco
Please note the chairs are firming the session description below:
Developments in techniques, including experimental methods, theory and simulation and specimen preparation. Topics include ultrahigh resolution SEM imaging, low voltage SEM, variable pressure SEM, transmission modes, channelling effects and contrast mechanisms in SEM, desktop SEM, cryo-SEM, FIB, including ion-solid interactions, techniques using high current, light ion or mass-filtered sources, low-energy milling and cryo-FIB for materials, LEEM, PEEM, AFM, including conductive, electrochemical and Kelvin probe, STM, MFM.
IT11 - Optical Nanoscopy and Spectral Imaging Techniques
Chairs: Colin Sheppard
Please note the chairs are firming the session description below:
Developments in techniques, including experimental methods, theory and simulation and specimen preparation. Super-resolution methods, PSF engineering, stochastic optical fluctuation imaging, FiM, STORM, PALM, STED, SIM etc fluorescence correlation and imaging spectroscopies (FCS, RICS, STICS, etc) and Fluorescence Lifetime imaging (FLIM). Joint sessions with LS -11. Innovations in Light / Laser Microscopy and Optical Nanoscopy will be considered.
IT12 - Spectroscopy – High energy excitations and local chemical analysis
Chairs: Gianluigi Botton & Gerald Kothleitner
Being able to measure, quantify and model various spectroscopic signals from different sources of radiation and instruments, has advanced the characterization of materials significantly. This wealth of information generates new grand challenges in understanding and modelling the underlying fundamentals of signal generation and intelligent processing of large data sets. The symposium will address the latest developments and technical advances in measuring, analysing and understanding high energy excitations in materials and chemical analysis with spectroscopies at high spatial resolution. Topics of interest will include light, electron, x-ray and other spectroscopies for measuring high energy excitations and for chemical analysis. This includes monochromated core-level EELS, EDX, WDS, EPMA as well as SE spectroscopy, low dose methods, atomic resolution chemical mapping, STXM, XAS, XAFS, XELFS, RIXS. Contributions are invited on hardware and technique developments, theory and simulation, data processing as well as applications covering both physical and biological sciences.
IT13 - Spectroscopy – Low energy excitations and ultrafast spectroscopy
Chairs: Odile Stephan & Javier Garcia de Abajo
Recent instrumental developments in electron microscopy, such as new aberration correctors, more efficient electron monochromators, or the ability of combining ultrafast electron-beam and light pulses, are opening a myriad of unprecedented opportunities, enabling the exploration of localized excitations over a broad spectral region (from the mid-infrared to the ultraviolet), with combined energy-space-time resolution that is quickly approaching the meV-nm-fs domain. The symposium will address the latest developments in combined light and electron spectroscopies for measuring, quantifying, understanding, and modelling a wide range of low-energy excitations of primary interest in various fields of applications, including elementary excitations in condensed matter systems; phonons and vibrational excitations in solids, nanoparticles, and molecules; and plasmons, excitons, and other types of polaritonic modes in optical materials. New findings in this field rely on an ample suite of techniques that include monochromated EELS, cathodoluminescence in TEM and SEM, time-resolved ultrafast spectroscopy, PEEM, LEEM, PINEM, SNOM, STM and AFM-based spectroscopies, RIXS, and electron/photon momentum-resolved spectroscopies.
IT14 – Advances in Atom Probe Tomography
Chairs: David Larson & Ross Marceau & Leigh Stephenson
APT is a powerful microscopy technique that provides 3D atomic-scale characterisation capability, and is increasingly finding application within diverse fields of materials research, including metals and alloys, semiconductor materials and devices, geological minerals, bio-materials and insulator (e.g. oxide) materials. In addition to spatial reconstruction of the 3D position and chemical identity of millions, tens of millions, and even hundreds of millions of atoms within these materials, APT offers associated capabilities such as time-of-flight mass spectrometry and field ion microscopy, as well as being highly suited for both complementary and direct correlative analysis with other microscopies, such as (but not limited to) TEM, SEM and FIB, and their respective ancillary techniques. Similar to APT, SIMS provides quantitative isotopic analysis with the extremely high sensitivity for all elements. Secondary ion emission by a solid surface under ion bombardment supplies information about the elemental, isotopic and molecular composition of its uppermost atomic layers and as such it is a highly sensitive surface analysis technique. This symposium welcomes all contributions related to advances in APT, including instrument developments, field evaporation theory and simulation, data processing, and current and emerging materials research applications, as well as contributions related to advances in SIMS.
Chair: Prof Julie Cairney – The University of Sydney, Australia
Committee: A/Prof Jodie Bradby, Prof Marc de Graef, Dr Alex la Fontaine, Prof Dr Joachim Mayer, A/Prof Matt Weyland, Prof Jin Zou
FI1 - Outreach
Chairs: Tim White & Jenny Whiting
The ability to see amazing detail in the invisible world holds enormous power to engage everyone, whether they are an experienced microscopist, young child, artist or from that broad-brush group, the ‘general public’. This outreach symposium seeks to highlight exciting and innovative projects, programs and activities that use microscopy to reach out beyond the usual core of microscopists and researchers. Examples could include hands-on activities, exhibitions, virtual and distance microscopy or anything else that uses the power of microscopy to get audiences excited about imaging and the STEM area in general.
FI2 - Data management (storage, processing and sharing)
Chairs: Wojtek Goscinski
A dramatic expansion in the volume of research data generated by microscopy and microanalysis is a result of both the increased richness or complexity of data that can be obtained from contemporary techniques, e.g. the increased digital and spectral resolution of modern detectors, and the transition from the probing systems in two- or three- dimensions toward the routine acquisition of 4D and 5D data arrays where compositional (spectroscopic) and time-resolved information are superimposed onto spatially resolved data sets. This leads to challenges with data storage processing and sharing. This symposium will compare approaches to solving these new problems.
FI3 - Facility management
Microscopy facilities may exist to serve a specific research effort, or they may be core user facilities that serve either a local or a wider range or researchers. This symposium will benefit any type of facility via the sharing of best practice in laboratory management, training procedures and safety practice.
Chair: A/Prof Filip Braet – The University of Sydney, Australia
Committee: Dr Lucy Collinson, Prof Yves Dufrêne, Dr Jan Ellenberg, Prof Katharina Gaus, A/Prof Eric Hanssen, Prof Bram Koster, Prof Thomas Müller-Reichert, Prof Rob Parton, Prof Peter Peters, Dr Melanie Rug, Dr Yannick Schwab, Prof Paul Verkade, Dr Renee Whan, Dr Rosemary White
Since the last IMC conference, the life sciences have witnessed unprecedented progress that coincides with the development of novel microscopy instrumentation and approaches. New breakthroughs in optical super-resolution imaging, cryogenic approaches, direct electron detection, including the ever-growing activity in correlative and integrated imaging have changed the landscape of biological microscopy for many generations to come. Contributors to these thrilling advances have recently been awarded the Nobel Prize in Chemistry for the invention of optical super-resolution imaging and cryogenic electron microscopy. This part of the IMC-19 program aims therefore to accurately report on those advances with special emphasis on how these innovations have contributed to our enriched understanding in the complexities of life.
LS-1. Structure and Function of Cells & Organelles
Chairs: Bram Koster & Sharon Wolf
Developments in techniques as well as their application to assess structure and function of cells and organelles. The sessions covers specimen preparation approaches, advances in multi-resolution multi-modal imaging methods and issues related to interpretation (e.g. on-section labelling, HPF/FS, in-resin fluorescence, chemical fixation, cryo-immobilization, SBF-SEM, array tomography, correlative microscopy, tomography, cryo tomography). The focus of the session is on the biological cell and its interactions, excluding structural studies of macromolecular assemblies.
LS-2. Multiplex Live Imaging of Cells, Tissues & Organisms
Chairs: Renee Whan
The capacity of light microscopy to reveal information in space and time has bourgeoned the development of technologies and applications for biological and biomedical research. Indeed temporal imaging is necessary to reveal and measure the complex dynamic information of biological systems. In this session we will focus on the use of live imaging; from 2 and 3-dimensional cell cultures to intravital and lightsheet microscopy. Particular focus will be on the capacity to examine multiple processes simultaneously, such as; multiscale or multi- molecular imaging, or correlating with spectral, lifetime, mechanical or structural components.
LS-3. 3-D Structures of Macromolecules & Supramolecular Assemblies
Chairs: Eric Hanssen & Gabriel Lander
Up until recently X-ray diffraction was the primary technique used to solve the structure of proteins of all sizes while electron cryo microscopy (cryoEM) was limited to large complexes and yielded a relatively low resolution. The release of a new generation of electron detectors along with phase plate and microscope automation is shifting the current paradigm. Single particle CryoEM can now rival synchrotron base x-ray sources in term of resolution without the need for crystallization. Other approaches such as Subtomogram Averaging can lead to near atomic resolution maps of protein within their natural environment while cryo Micro Electron Diffraction yield atomic resolution structures in a few minutes of acquisition. This session will cover recent advances and illustrate the field of opportunities that cryoEM can offer the structural biology community.
LS-4. Atomic Force Microscopy in Molecular and Cell Biology
Chairs: Yves Dufrêne & Peter Hinterdorfer
Within three decades, atomic force microscopy (AFM) has established as a powerful multifunctional probing platform in biology, enabling the imaging and manipulation of biological samples down to molecular resolution. Achieving the full potential of AFM in biology has required continuous technological developments in order to improve important limitations of the method. This session will highlight recently developed bio-AFM modes, including multiparametric, molecular recognition, and high-speed imaging, as well as advanced force spectroscopy assays for measuring interactions in single molecules and cells. Invited and contributed talks will emphasize the enormous potential of AFM in fields ranging from structural and molecular biology to microbiology and cell biology.
LS-5. Cellular Transport & Dynamics
Chairs: Rob Parton & Georg Ramm
Research into the inner workings of the cell and the dynamic biological complexes required for life is being revolutionised by the application of a newly developed suite of microscopic techniques. This session will highlight some of the new methods being applied to the understanding of cellular organization with a particular emphasis on membrane transport and intracellular dynamics. This session will cover a range of techniques, including both advanced light microscopy and new methods in electron microscopy, and their application to the study of intracellular organisation and dynamic cellular processes.
LS-6. Applications of Cryo Electron Microscopy in Biology
Chairs: Thomas Mueller-Reichert & Michel Steinmetz
This session covers recent advances and applications in biological cryo-electron microscopy (cryo-EM) at the molecular, cellular and organismic level. This session welcomes all contributions in which cryo-EM approaches made a significant contribution to address a particular biological question that could not be undertaken using conventional scanning- and/or transmission electron microscopy approaches. In addition, aspects of 3D data collection and data modelling obtained under cryo-imaging conditions such as electron tomography and FIB-SEM will be covered. This session excludes molecular imaging by cryo-EM for single particle analysis.
LS-7. Embryology & Developmental Biology
Chairs: Louise Cole & Anastasios Pavlopoulos
From early embryological descriptions to contemporary systems-level developmental reconstructions, many breakthroughs in embryology and developmental biology have been intrinsically linked to advances in microscopy. This session will cover recent progress made in noninvasive in vivo live-imaging technologies, including state-of-the-art microscopy modalities, labeling strategies, and computational image analysis tools. These improvements have enabled to meet the challenges of capturing and measuring the behaviors of thousands of cells, subcellular structures or even single molecule dynamics in their normal context of development. Entire embryos can be visualized throughout their development at high spatiotemporal resolution and with minimal photo-toxicity leading to fundamental insights into the molecular, cellular and biophysical mechanisms underlying cell fate specification, tissue and organ morphogenesis and differentiation.
LS-8. Pathology and Immunocytochemistry & Biomolecular Labeling
Chairs: Danielle Jorgen
The localisation of proteins and structures of interest is of critical importance in Biomedical Research. There is a whole plethora of techniques and approaches to try to achieve maximum labelling, best penetration and optimal visualisation. This can be done using pre- and post-embedding methods using both particulate particles and precipitation methods. In this session we will cover both novel labelling strategies as well as new or innovative ways of using established methods.
LS-9. Applications in Correlative Microscopy of Biological Systems
Chairs: Lucy Collinson & Yannick Schwab
Correlative microscopy is a growing family of techniques that combines, on one sample, a sequence of various imaging modalities, opening to structure-function analysis in the Life Sciences. While covering a range of methodological implementations on various model systems this session will also focus on examples of ground-breaking applications in Biology. We would welcome contributions that promote correlating any of such imaging modalities (but not restricted to): fluorescence imaging, electron microscopy, cryo-EM, volume EM, micro-CT, soft X-ray, AFM, etc.
LS-10. Plant Science & Mycology
Chairs: Rosemary White & Staffan Persson
This session will focus on all aspects of plant and fungal cell biology, including analysis at the tissue and organ level. The key will be use of new technologies and preparation techniques to obtain novel image data to understand cell and tissue composition, growth, and dynamics. This can include tissue and cellular localization of essential nutrient elements, composition and dynamics of plant and fungal structures and organelles, interactions with the environment and plant-fungal interactions.
LS-11. Innovations in Light / Laser Microscopy and Optical Nanoscopy
Chairs: Jan Ellenberg
This session explores the recent technological advances in light microscopy and super-resolution fluorescence microscopy and their applications in biology. These imaging technologies are anticipated to revolutionise cell and molecular biological as they bridge the resolution gap between electron microscopy with nanometre and sub-nanometre resolution and conventional light microscopy that typically has a resolution limit of several hundreds of nanometres. The session will include research in instrumentation and improved data acquisition particularly for biological samples as well as novel approaches in image and data analysis to extract biologically meaningful information
LS-12. Multimodal Molecular Imaging in Health & Disease
Chairs: Peter Peters & Peijun Zhang
Electron cryo tomography has brought an explosion of new mechanistic insights into the biology in health and disease. Therefore this session will focus on new studies of macromolecular assemblies inside intact cells. Often such machines cannot be purified or the structure is lost outside the cell, so that the mechanism can be understood only by observation in situ. In this session, we will also highlight method developments that will improve and expand the scope three-dimensional views of macromolecular complexes and we will provide examples of its power when applied to cell biology. Mass Spec Imaging, Serial Block Face Scanning, Focused Ion Beam Milling are revealing new insights that feed electron cryo tomography. In this session we will welcome and highlight the latest developments of multimodel macromolecular imaging in health and disease.
LS-13. Invertebrate Biology & Taxonomy
Invertebrates comprise many different forms of life. Some are established model organisms such as Drosophila and C elegans that are widely used to study various aspects of life. Others are studied for their medical importance, such as parasitic helminths. Their taxonomy has always been reinforced by microscopical analyses. Nowadays we witness a revival of ultra-microscopy studies to tackle the classification of fast growing numbers of newly discovered organisms, in particular of marine organisms. This symposium will highlight recent fascinating examples of advanced light and electron imaging of various invertebrates, including marine organisms, embryonic development, origin and evolution of nervous system.
LS-14. Host-Pathogen Interactions, Microbiology & Virology
Chairs: Melanie Rug
Parasitic organisms, microbes and viruses are the causing agents of major diseases and are accountable for millions of deaths around the world each year. In order to combat these masters of adaptation and evolution we need to understand the intricate relationships with their host cells and tissues. This session covers advanced methods in microscopy ranging from studying dynamics of host-pathogen interactions in artificial membranes to elucidating their complex development and interactions by superresolution and 3D cryo electron microscopy techniques.
Please note the above information is correct as of 18 January 2018 and is subject to change.
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