Workshops

Workshops available to registered Congress delegates and will be held on Saturday 8 September and Sunday 9 September 2018. Minimum and maximum numbers apply to all workshops. Those workshops for which minimum numbers are not met may be cancelled and delegates will be notified via email. Workshops will be allocated on a first come first served basis.

Please note: Attendance to the workshops is not included in your registration fee. Maximum numbers apply to all workshops and will be allocated on first come first served basis. 

Saturday 8 September 2018 – University of Sydney

WS1 - EM Maintenance

Speakers/Facilitators: Dr Richard Wurher
Location: University of Sydney, Madsen Building, room TBA
Date: Saturday 8 September 2018
Time: 0900 – 1700
Cost: $150.00

Program:
The care and correct maintenance of an SEM is the key aspect for a correctly operating instrument. This workshop will cover many aspects of SEM maintenance. Some of the topics to be covered include: Vacuum Systems and their maintenance, cleaning of microscopes parts such as apertures and wehnelt assembly, basic monitoring of instrument, testing of instrument operation (resolution, magnification calibration), EDS maintenance and calibration, monitoring filament conditions (filament life improvement and setting), and sorting out imaging problems.

Participants will be required to make their own transport arrangements to USyd. For information on public transport options, please see https://transportnsw.info

Minimum: 5
Maximum: 30

WS2 - Multi Dimensional Visualisation and Analysis

Speakers/Facilitators: Cameron Nowell (Monash) & Mike Carnell (UNSW)
Location: University of Sydney, Madsen Building, room TBA
Date: Saturday 8 September 2018
Time: 1300-1700
Cost: $75.00

Program:
A half day Master class with hands on components covering complex multi-dimensional analysis. Attendees will be taken through the rationale and steps required to analyse movement of macro objects such as worms, track and measure residency time from TIRF data and measure 3D diffusion. All examples will be carried out using the Fiji distribution of ImageJ. Attendees will need to bring along a laptop and have Fiji installed.

Participants will be required to make their own transport arrangements to USyd. For information on public transport options, please see https://transportnsw.info

WS3 - Multiplex Immunohistochemistry

Speakers/Facilitators: A/Prof Sarah Ellis (Peter MacCallum Cancer Centre, Melbourne) &  Dr Sandrine Roy (UQ)
Location: University of Sydney, CPC, room TBA
Date: Saturday 8 September 2018
Time: 0900 – 1300
Cost: $75.00

Program:
This half day workshop will focus on techniques for sample preparation for mIHC and compare different imaging methods using multispectral imaging systems Perkin Elmer Vectra/Mantra and Confocal Microscopy with spectral imaging. There will be a demonstration of Inform software for image analysis. There will be a demonstration of current advanced techniques coupling immunohistochemical methods with high-resolution CyTOF mass cytometry.

Participants will be required to make their own transport arrangements to USyd. For information on public transport options, please see https://transportnsw.info

Minimum: 5
Maximum: 30

WS4 - Raman and Infrared Microscopy

Speakers/Facilitators: Dr Elizabeth Carter (University of Sydney) & Dr Richard Wuhrer (University of Western Sydney)
Location: University of Sydney, Madsen Building, room TBA
Date: Saturday 8 September 2018
Time: 0900 – 1700
Cost: $150.00

Program:
Raman and infrared spectroscopy are non-destructive techniques that are used for the identification and characterisation of materials on a micron and more recently, sub-micron scale. Mapping and imaging provides information about the identity, size, location and distribution of components within a sample without the need for external chemical markers. The workshop will be split into two ½ day sessions with the morning devoted to providing a general introduction to Raman and infrared (IR) spectroscopy with emphasis on the sampling techniques of mapping and imaging. The afternoon session will be more advanced offering the opportunity for hand-on data collection with state-of-the-art equipment located at the Vibrational Spectroscopy Core Facility at the University of Sydney.

Participants will be required to make their own transport arrangements to USyd. For information on public transport options, please see https://transportnsw.info

Minimum: no minimum 
Maximum: 36

WS5 - EBSD

Speakers/Facilitators: Dr Vijay Bhatia (USYD)
Location: University of Sydney, Madsen Building, room TBA
Date: Saturday 8 September 2018
Time: 1300-1700
Cost: $75.00

Program:
This course will go through a short introduction to EBSD and some of the common set up requirements for different applications. We will then go into more advanced techniques. The topics that will be covered include and introduction to crystallography, sample preparation, introduction to EBSD (set up and configuration), Advanced techniques – TKD, strain analysis, in-situ and ECCI. There will also be an open discussion panel for attendees to ask questions.

Participants will be required to make their own transport arrangements to USyd. For information on public transport options, please see https://transportnsw.info

Minimum: 5
Maximum: 30

WS6 - Live Cell Microscopy

Speakers/Facilitators: Yingying Su (ACMM) and Robert Woolley (Coherent)
Location: University of Sydney, Madsen Building, room TBA
Date: Saturday 8 September 2018
Time: 0900 – 1300
Cost: $75.00

Program:
Advanced Live Cell Microscopy using NIS Elements JOBS This workshop is aimed at Nikon Live Cell Users who have the JOBS module and are interested in learning how to design and operate experiments with JOBS. Many experiments would benefit from wizard based set-up tools or advanced and conditional image acquisition and on the fly data analysis to dictate such. This is especially true of hypoxia studies, photo activation and conversion studies, screening and many others. This workshop will use case studies to discuss how to use triggering in JOBS with general analysis to achieve highly advanced imaging experiments.

Participants will be required to make their own transport arrangements to USyd. For information on public transport options, please see https://transportnsw.info

Minimum: 8
Maximum: 12

WS7 - Helium Ion

Speakers/Facilitators: Steve Moody, John Notte, Annalena Wolff & more TBA
Location: University of Sydney, Madsen Building, room TBA
Date: Saturday 8 September 2018
Time: 0900 – 1700
Cost: $150

Program:
The Helium Ion Microscope (HIM) is an exciting new technology with a vast range of applications from materials characterization to nanostructuring. Up to three different ion species helium, neon and gallium can be selected and allow the HIM to excel at high resolution surface imaging of conductive and non-conductive samples such as polymers and uncoated biological samples while adding structuring capabilities from the micron to sub 10nm range. The workshop will cover all essential aspects of Helium ion Microscopy, starting with the history of the HIM and an easy to understand overview of the Helium Ion Microscope technology and it’s vast range of applications. The workshop will then focus on the underlying GFIS and column technology, the ion solid interactions and the resulting applications. Topics which have been identified as topics of special interest by the attendees will then be covered by experts in the field. The workshop will provide the attendees with a ‘need to know overview of Helium Ion Microscopy’.

Participants will be required to make their own transport arrangements to USyd. For information on public transport options, please see https://transportnsw.info

Minimum: 5
Maximum: 16

WS9 - Quantifying, and understanding, colocaisation

Speakers/Facilitators: Michael Carnell (UNSW)
Location: University of Sydney, Madsen Building, room TBA
Date: Saturday 8 September 2018
Time: 1300 – 1700
Cost: $75.00

Program:
One of the strengths of fluorescence microscopy is the ability to image multiple structures of interest within the same sample. However, interpreting how these signals spatially relate to each other, and also quantifying these similarities or differences, can be challenging. In this workshop we will give practical guidance on designing colocalisation based experiments, but focus primarily on analysis. We hope to impart useful guidelines and instill an appreciation for the various approaches available. In doing so you will be equipped to understand what measurements are most applicable in various situations, and better justify your observations.

Participants will be required to make their own transport arrangements to USyd. For information on public transport options, please see https://transportnsw.info

Minimum: 5
Maximum: 25

Saturday 8 September 2018 – University of Technology, Sydney

 

WS10 - Scanning Cathodoluminescence Microscopy

Speakers/Facilitators: Matthew Phillips & Katie McBean (UTS)
Location: University of Technology, building and room TBA
Date: Saturday 8 September 2018
Time: 0900 – 1300
Cost: $75.00

Program:
Scanning cathodoluminescence (CL) microscopy and spectroscopy currently plays an important role in the optical, electrical and chemical characterisation of bulk and nano-structured semiconductor materials and devices, ceramics as well as geological materials at the sub-micron level. This workshop will cover; CL generation mechanisms and instrumentation, CL experimental techniques and analysis methods, including depth-, power density- and temperature-resolved CL analysis, approaches used to identify and assign CL emission peaks, spectral CL imaging, as well as the current applications of CL in semi-conductor nanophotonics, nano-technology, and the geo- and biosciences.

Participants will be required to make their own transport arrangements to UTS. For information on public transport options, please see https://transportnsw.info

Minimum: no minimum
Maximum: no maximum

Saturday 8 September 2018 – University of Wollongong

WS11 - Cryo- Electron Microscopy

Speakers/Facilitators: Steve Ludtke (Baylor College of Medicine, NCMI, CIBR ), Gabe Lander (The Scripps Research Institute), Jose Rodrigues (University of California, Los Angeles) Michael Landsberg (University of Queensland), Eric Hanssen (University of Melbourne), Hans Elmlund (Monash University) and James Bouwer (University of Wollongong).
Location: Main campus, University of Wollongong, building and room TBA
Date: Saturday 8 September 2018
Time: 0900 – 1700
Cost: $150.00

Program:
This is a hands-on workshop on cryo-electron microscopy covering a wide range of technical aspects. These aspects include setting up and performing automated cryo-electron microscopy for both single particle and tomographic workflows. The workshop will cover sample preparation, low-dose strategies, direct detection devices (DDDs), remote microscope operation, and data collection and processing strategies. Attendees will have the opportunity to run a state of the art K2 and Falcon3 equipped energy-filtered FEI Krios microscope through remote console. Join us for a remarkable bus tour into the heart of the beautiful Illawarra coastal valley. The workshop will run for 6 hours and highlight key insights into the cryo-electron microscopy workflow. All levels of experience are welcome

Minimum: 10
Maximum: 30

WS12 - Electron Energy Loss Spectroscopy

Speakers/Facilitators: Associate Professor Vicki Keast (UON) , Dr David Mitchell (UOW)
Location: University of Wollongong Electron Microscopy Centre
Date: Saturday 8 September 2018
Time: 0900 – 1700
Cost: $150.00

Program:
The Electron Microscopy Centre, University of Wollongong, will offer lecture-style (for unexperienced microscopists, ½ day) and hands-on (for experienced microscopists, full day, limited to 15) workshop on Electron Energy Loss Spectroscopy (EELS). The workshop will cover the basic principles, applications and advanced EELS technique. Demonstrations using aberration-corrected cold field emission gun scanning transmission electron microscope ARM 200F will be conducted in addition to data analysis and simulations sessions.  Join us for a memorable bus tour into the heart of the beautiful Illawarra coastal valley.  

Minimum: 10
Maximum: 16

Saturday 8 September 2018 – University of NSW

WS13 - Focused Ion Beams: Principle and Application

Speakers/Facilitators: Charlie Kong (UNSW)  

Location: University of New South Wales, Building and room TBA

Date: Saturday 8 September 2018

Time: 0900 – 1700

Cost: $150.00

Program:

Focused ion beams (FIB) and associated techniques have been developed as an essential multifunctional platform for nanofabrication and characterisation in multidisciplinary academic areas, including microelectronics, renewable energy, advanced materials sciences and engineering, mechanical engineering, geosciences, and biological applications etc. This workshop will include the introduction to FIB fundamentals and a number of case studies on its practical applications to both physical sciences and life sciences. It is suitable for both new starters and experienced FIB users.

Participants will be required to make their own transport arrangements to UNSW. For information on public transport options, please see https://transportnsw.info

Minimum: 10

Maximum: 49

Sunday 9 September 2018 – University of Sydney

WS15 - Intravital Imaging in Cancer and Immunology

Speakers/Facilitators: Paul Timpson
Location: University of Sydney, Madsen Building, room TBA
Date: Sunday 9 September 2018
Time: 0900 – 1700
Cost: $150.00

Program:
This one-day workshop you will cover both practical and analytical aspects of intravital imaging. It will consist of small groups of 5 watching 2 types of intravital imaging. 1) Cancer and FLIM-FRET and 2) single cell imaging of immune cells. A session will also be included demonstrating relavent analysis technqiues for the practical sessions demonstrated.

Participants will be required to make their own transport arrangements to USyd. For information on public transport options, please see https://transportnsw.info

Minimum: 5
Maximum: 25

WS16 - A Practical Introduction to Simulation and Analysis Software in Atomic Resolution S/TEM

Speakers/Facilitators: Dr Scott Findlay (Monash University) & Dr Hamish Brown (Monash University)
Location: University of Sydney, Madsen Building, room TBA
Date: Sunday 9 September 2018
Time: 0900 – 1700
Cost: $150.00

Program:
This full-day workshop overviews some software suites for processing and analysing imaging, diffraction and spectroscopic data recorded in atomic-resolution S/TEM and for performing supporting simulations. Participants will practise running simple simulations and analyses with them. Software suites include:
• HyperSpy: open-source, Python-based software written by a community of microscopists for analysing multidimensional data (including spectral data and scanning diffraction).
• SmartAlign: a tool for alignment, drift- and distortion-correction of images to maximise resolution, precision and signal-to-noise.
• StatSTEM: a program for accurate and precise model-based quantification of atomic-resolution S/TEM images.
• μSTEM: open-source, Fortran-based software for image simulation in S/TEM (including HAADF and EDX).

Participants will be required to make their own transport arrangements to USyd. For information on public transport options, please see https://transportnsw.info

Minimum: 12
Maximum: 50

WS17 - Volume SEM for Life Sciences

Speakers/Facilitators: Mr Rick Webb (UQ) & Dr Minh Huynh (USyd)
Location: University of Sydney, Madsen Building, room TBA
Date: Sunday 9 September 2018
Time: 0900 – 1700
Cost: $150.00

Who should attend?
This workshop is suitable for experienced SEM biological users.

Program:
This workshop will give an overview of volume scanning electron microscopy (SEM) techniques currently available for life sciences. There will be a series of lectures and demonstrations which will cover sample preparation for biological samples including automated serial sectioning, serial block face SEM (SBF SEM), array tomography, focussed ion beam SEM (FIB SEM) and correlation of volume SEM with light and X-ray microscopy.

Participants will be required to make their own transport arrangements to USyd. For information on public transport options, please see https://transportnsw.info

Minimum: 9
Maximum: 15

WS18 - Atom Probe

Speakers/Facilitators: Dr Hugues Francois-Saint-Cyr (Applications Scientist, CAMECA, AMETEK Materials Analysis Division),
Dr David Saxey (Senior Research Fellow, Geoscience Atom Probe Facility Manager, Curtin University), Dr Ross Marceau (Senior Research Fellow, Advanced Characterisation, Institute for Frontier Materials, Deakin University), Dr Takanori Sato (Advanced Atom Probe Microscopy Engineer, Australian Centre for Microscopy & Microanalysis, The University of Sydney).

Location: University of Sydney, Madsen Building, room TBA
Date: Sunday 9 September 2018
Time: 0900 – 1500
Cost: $150.00

Program:
This tutorial-style workshop will focus on the introduction of the atom probe technique to new and potential users. The primary goal of the workshop is to encourage a wider area of advanced microscopy and scientific community to become familiar with the recent advances in the atom probe technique that enables nano/atomic scale 3D characterisation on a broad range of materials.

Some of the key topics covered:
General introduction to the atom probe technique, nano/atomic scale 3D imaging and chemical composition analysis, specimen preparation requirements.
Overcoming the past challenges and expanding the application fields with the latest advances in the atom probe instrumentation.
Recent progresses in 3D reconstruction based on correlative microscopy, in particular using nanoSIMS, EBSD/TKD and TEM.

This workshop is suitable for those who are new to APT.

Participants will be required to make their own transport arrangements to USyd. For information on public transport options, please see https://transportnsw.info

Minimum: no minimum
Maximum: 20

WS19 - X-ray Microscopy

Speakers/Facilitators: Dr Matt Foley (University of Sydney)
Location: University of Sydney, Madsen Building, room TBA
Date: Sunday 9 September 2018
Time: 1300 – 1700
Cost: $75.00

Program:
This half day workshop will provide an introduction to X-ray Microscopy for both novice and intermediate level researchers with presentations from experts in both lab and synchrotron based equipment. Planned topics include different imaging modalities such as phase contrast and x-ray fluorescence, as well as experimental design considerations and artefact correction methodologies. Real-world case studies will be presented to show how XRM is being applied in research today.

Participants will be required to make their own transport arrangements to USyd. For information on public transport options, please see https://transportnsw.info

Minimum: no minimum 
Maximum: 24

Sunday 9 September 2018 – University of Technology, Sydney

 

WS20 - Nanofabrication Using Focused Electron and Ion Beams

Speakers/Facilitators: Prof Milos Toth (University of Technology Sydney) & Katie McBean (University of Technology Sydney)
Location: University of Technology Sydney, building and room TBA
Date: Sunday 9 September 2018
Time: 1300 – 1700
Cost: $75.00

Program:
Overview of beam-directed techniques for material deposition, etching/milling and surface functionalization. The workshop will be focused on the benefits and limitations of electron, Ga FIB and plasma FIB techniques in the context of resolution, throughput and damage to devices and functional materials. It will also cover recent applications of the techniques, with an emphasis on optoelectronic materials and 2D materials.

Participants will be required to make their own transport arrangements to UTS. For information on public transport options, please see https://transportnsw.info

Minimum: no minimum 
Maximum: no maximum

Sunday 9 September 2018 – University of New South Wales

WS21 - Electron Probe Microanalysis

Speakers/Facilitators: Dr Karsten Goemann (University of Tasmania) & Dr Karen Privat (University of NSW)

Location: University of NSW, building and room TBA

Date: Sunday 9 September 2018

Time: 0900 – 1700

Cost: $150.00

Program:

This workshop is designed to cater for users with a basic familiarity with SEM-based microanalysis, right up to advanced operators. It will cover fundamentals as well as advanced topics of electron microprobe analysis (EPMA), such as WDS-EDS integration, low kV analysis, dealing with beam sensitive materials, trace and complex material analysis, background and interference corrections, quantitative mapping, sample preparation, standards, and software options. Real world application examples will be integrated into the program.

Contributions and participants from all areas of research and industry are encouraged; please contact the organisers to discuss.

Participants will be required to make their own transport arrangements to UNSW. For information on public transport options, please see https://transportnsw.info

Minimum: 8

Maximum: 30

WS22 - Introduction to Advanced Atomic Force Microscopy (AFM) Techniques

Speakers/Facilitators: Dr Yin Yao (University of NSW), Dr Chris Gibson (Flinders University), Wojciech Chrzanowski (University of Sydney) & Celine Heu (University of NSW).

Location: University of NSW, building and room TBA

Date: Sunday 9 September 2018

Time: 0900 – 1700

Cost: $150.00

Who should attend?

This is designed for both new and intermediate level AFM users. Anyone who is interested in AFM related techniques, both in biological and physical sciences are welcome.

Program:

This workshop will aim to cover both the basic principles of atomic force microscopy as well as advanced imaging modes, including: peakforce quantitative nanomechanical measurements, electrical characterisation methods and TERS (AFM-Raman spectroscopy). There will be a brief explanation of these techniques using case studies provided by guest speakers. The application of these methods for both physical and life sciences will also be discussed. The workshop will include demonstrations to showcase some of these AFM methods using the Bruker ICON and the JEOL 5400 MK II SPM. Furthermore, the workshop will dedicate a session to discussing the challenges relating to image of biological materials using AFM.

Participants will be required to make their own transport arrangements to UNSW. For information on public transport options, please see https://transportnsw.info

Minimum: 5

Maximum: 40

WS23 Correlative Light and Electron Microscopy

Speakers/Facilitators: Dr Nicholas Ariotti (University of NSW) & Dr Ben Padman (Monash University)

Location: University of NSW, building and room TBA

Date: Sunday 9 September 2018

Time: 0900 – 1700

Cost: $150.00

Who should attend?

This workshop will be suitable for both experienced and inexperienced biological microscopists.

Program:

This workshop will provide an overview of the currently available correlative light and electron microscopy (CLEM) techniques for the biological sciences. The workshop will encompass a series of lectures around advances in CLEM-based methodologies including super-resolution fluorescence microscopy, enzymatic-tagging methods for correlation and improved specimen registration. The workshop will end with a series of demonstrations of varied sample preparation techniques for correlative electron microscopy including high pressure freezing as well as computational sample registration.

Participants will be required to make their own transport arrangements to UNSW. For information on public transport options, please see https://transportnsw.info

Minimum: 10

Maximum: 49

WS24 - Fluorescence Fluctuation Analysis of Intracellular Protein Dynamics

Speakers/Facilitators: Dr Elizabeth Hinde (University of NSW) & Dr Elvis Pandzic (University of NSW)

Location: University of NSW, building and room TBA

Date: Sunday 9 September 2018

Time: 0900 – 1300

Cost: $75.00

Program:

In this workshop, we first present the basic principles behind fluorescence fluctuation analysis on a confocal (or 2-photon) microscope. We share how this information can be used to extract the concentration, co-localization, oligomeric state and diffusion coefficient of proteins inside the cell. Then we will review how fluorescence fluctuation analysis across a line or frame scan (spatio-temporal correlation spectroscopy) can extract the diffusive route and flow maps that proteins adopt in the context of live cell architecture. Finally, we will demonstrate this technology in a living cell on a confocal microscope within the Biomedical Imaging Facility, University of New South Wales and teach participants how to analyse the data to quantify and extract the relevant parameters for proteins under study.

Participants will be required to make their own transport arrangements to UNSW. For information on public transport options, please see https://transportnsw.info

Minimum: 10

Maximum: 20

WS25 - Fluorescence Lifetime Imaging Workshop

Speakers/Facilitators: Dr Alex Macmillan (University of NSW) & Dr Kirsten Elgass (Monash University)

Location: University of NSW, building and room TBA

Date: Sunday 9 September 2018

Time: 1300 – 1700

Cost: $75.00

Program:

Practical sessions will be divided into multiple topics. Firstly the focus will be on sample preparation and data acquisition. Like in any microscopy if the sample or the microscope is not set up properly the data will not make any sense. We will concentrate on common pit falls in fluorescence lifetime measurement including the correct selection of laser repetition rate for lifetime experiments and avoiding pulse pile-up effects. We will cover the most common applications of FLIM including measurements of autofluorescence and its applications, we will demonstrate the effect of environment on fluorophore lifetime (polarity, pH, solvent etc), and Fluorescence resonance energy transfer (FRET) and Biosensors. In the second part of the practical session we will focus on data analysis. We will cover correct decay fitting, phasor analysis, and also pattern matching analysis. In this section we will cover the principles of the analysis methods and look at some real applications.

Participants will be required to make their own transport arrangements to UNSW. For information on public transport options, please see https://transportnsw.info

Minimum: 10

Maximum: 20

WS26 - Systems Microscopy: systems biology resolved in time and space

Speakers/Facilitators: John Lock (UNSW), Sarah Russell (PeterMacCallum Cancer Centre)

Location: University of New South Wales, Building and room TBA

Date: Sunday 9 September 2018

Time: 0900 – 1300

Cost: $75.00

Program:

Systems Microscopy is a rapidly emerging research strategy that combines the spatiotemporal resolving power of microscopic imaging with quantitative, systems-oriented analytical approaches from systems biology. This workshop will introduce the Systems Microscopy concept and its practical application, highlighting key features of this strategy. These include experimental design; experimental and imaging automation; quantitative image analysis; statistical interrogation and inference methods including machine learning, as well as; data visualisation and communication. Field-leaders spanning this multidisciplinary strategy will describe and integrate these features in the context of their research, driving a discussion of current Systems Microscopy capabilities and its future directions.

Participants will be required to make their own transport arrangements to UNSW. For information on public transport options, please see https://transportnsw.info

Minimum: 10

Maximum: 40

 

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